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A convenient and rapid method for the selective oxygen-17 enrichment of aspartyl peptides during solid-phase synthesis

✍ Scribed by Vassiliki Theodorou-Kassioumis; Nikolaos Biris; Constantinos Sakarellos; Vassilios Tsikaris

Publisher
Elsevier Science
Year
2001
Tongue
French
Weight
137 KB
Volume
42
Category
Article
ISSN
0040-4039

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✦ Synopsis

In this work we describe, for the first time, a rapid and efficient method for 17 O selective labeling on the b-carboxyl group of an aspartic acid residue already incorporated into a peptide sequence anchored on a solid-phase support. The b-O-benzyl ester of the Asp residue of the Ac-RGD-benzydrylamine resin was successfully saponified using Na 17 OH in a methanol/ dichloromethane mixture. The 17 O selective enriched peptide was then released from the solid support by acidic cleavage. The 17 O NMR spectrum confirmed the 17 O labeling of the Asp b-carboxylate.

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