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A continuous thermal lysis procedure for the large-scale preparation of plasmid DNA

✍ Scribed by Kaichun Zhu; Huali Jin; Yijie Ma; Zhihui Ren; Chong Xiao; Zhonghuai He; Fuchun Zhang; Qinghong Zhu; Bin Wang

Book ID
118471348
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
143 KB
Volume
118
Category
Article
ISSN
0168-1656

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πŸ“œ SIMILAR VOLUMES

A simple procedure for large-scale prepa
A simple procedure for large-scale preparation of pure plasmid DNA free from chromosomal DNA from bacteria
✍ M. Mukhopadhyay; N.C. Mandal πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 870 KB

A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu