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Current Protocols in Molecular Biology || Large-Scale Preparation of Plasmid DNA

✍ Scribed by Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin

Book ID
120020237
Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
202 KB
Volume
41
Category
Article
ISBN-13
9780471142720
ISSN
1934-3639

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Current Protocols in Molecular Biology |
Current Protocols in Molecular Biology || Introduction of Plasmid DNA into Cells
✍ Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidm πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley & Sons, Inc. βš– 146 KB

## Abstract Transformation of __E. coli__ can be achieved using any of the four protocols in this unit. The first method using calcium chloride gives good transformation efficiencies, is simple to complete, requires no special equipment, and allows storage of competent cells. The alternate one‐step