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A continuous acetie acid system for polyacrylamide gel electrophoresis of gliadins and other prolamines

✍ Scribed by Dr. Robert L. Clements

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 473 KB
Volume: 9
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150090206

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✦ Synopsis

A polyacrylamide gel electrophoresis system buffered by acetic acid alone was developed for electrophoresis of prolamines. When applied to gliadin electrophoresis, the acetic acid system produces more bands than does a conventional aluminum lactate-lactic acid system (using 12% acrylamide gels). The acetic acid system is relatively simple, requiring a single buffer component that is universally available in high purity.

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