A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulfate-containing polyacrylamide gels
β Scribed by Joseph J. Lanzillo; Joanne Stevens; Barry L. Fanburg
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 748 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0173-0835
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Some Aspects of the Use of "Continuous" and "Discontinuous" Buffer Systems in Polyacrylamide Gel Electrophoresis STELLAN HJERTBN, STURE JERSTEDT, AND ARNE TISELIUS From the Iustilute o,f Biochemistry, U,liucssity o( l'ppsala,
A chromogenic substrate autography is described which allows characterization and quantification of serine proteases in crude systems after sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing. Separation of samples containing proteases by either method is followed by an