## Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. P
✦ LIBER ✦
Some aspects of the use of “continuous” and “discontinuous” buffer systems in polyacrylamide gel electrophoresis
✍ Scribed by Stellan Hjertén; Sture Jerstedt; Arne Tiselius
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 717 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Some Aspects of the Use of "Continuous" and "Discontinuous" Buffer Systems in Polyacrylamide Gel Electrophoresis STELLAN HJERTBN, STURE JERSTEDT, AND ARNE TISELIUS From the Iustilute o,f Biochemistry, U,liucssity o( l'ppsala,
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## Abstract Using a horizontal slab apparatus with a buffer in the reservoirs at the level of the gel (“sea‐level electrophoresis”), the retrograde discontinuous buffer system reported by Wiltfang __et al.__ for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of proteins was ap