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A Consistent Inoculum System to Study the Fermentation of Non-starch Polysaccharides

✍ Scribed by Stevenson, A; Buchanan, C J; Eastwood, M A

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
231 KB
Volume
73
Category
Article
ISSN
0022-5142

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✦ Synopsis

A variable in batch culture fermentation systems for studying the fermentation of polysaccharides is the faecal inoculum. A commercial bacterial bead preservation system (MicrobankTM) has been used for the long-term storage of faecal inoculum. The inoculated beads are stored at [70¡C for up to 3 months and retain their ability to ferment pectin, as measured by production of short-chain fatty acids. A recovery medium is used for 27 h followed by culture in a standard medium for 48 h. Such a system o †ers a signiÐcant advantage over the present method of using fresh faeces on each occasion both in terms of ease of use and consistency of the inoculum. This allows for repeatable fermentation experiments over prolonged periods of time.

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✍ Agnes Stevenson; Callum J Buchanan; Martin A Eastwood 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 481 KB

## Abstract It is possible to anticipate the action of dietary non‐starch polysaccharides (NSP) using in‐vitro methods. The mode of isolation of hydrated polysaccharide suspensions previous to analysis may influence the result. A commercial hydrated polysaccharide (Frucht and Faser) was isolated us