๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A computerized system for measuring cell length of single isolated smooth muscle cells

โœ Scribed by Chafiq Moummi; Mark Woodford

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
277 KB
Volume
25
Category
Article
ISSN
0272-4391

No coin nor oath required. For personal study only.

โœฆ Synopsis

We have developed a state-of-the-art computerized system to measure the cell length (contractionirelaxation) of single isolated gastrointestinal smooth muscle cells in response to different drugs. The system is composed of a microscope to which a video camera is attached and a video monitor which is connected to the video camera output. The image on the TV screen is analyzed by a digital image processor. The data acquisition and initial analysis are done directly on a personal computer. The acquired data are sent immediately via a network to a database on a minicomputer. From this database, comprehensive data reports and graphs can be generated and hard copy obtained. The system was validated by testing the contractile activity of endothelin-1 and the relaxant effect of isoproterenol on single smooth muscle cells isolated from the rat colon. This system offers a valuable pharmacological tool for drug screening to the pharmaceutical industry.

๐Ÿ“œ SIMILAR VOLUMES

A magnetic colloid system for isolation
A magnetic colloid system for isolation of rare cells from blood for FISH analysis
โœ Bill Martin; Jenny Bruce; Nigel Smith; David Liu; Lindy Durrant ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 87 KB ๐Ÿ‘ 1 views

To improve cell recovery of trophoblast and nucleated red blood cells from maternal blood for diagnosis of chromosomal abnormalities, we have investigated the use of a magnetic sorting system that utilizes a ferrofluid. The main advantage of this system is that the beads used are small enough to all

DEVELOPMENT OF A MODEL SYSTEM TO COMPARE
DEVELOPMENT OF A MODEL SYSTEM TO COMPARE CELL SEPARATION METHODS FOR THE ISOLATION OF FETAL CELLS FROM MATERNAL BLOOD
โœ DIANA W. BIANCHI; KATHERINE W. KLINGER; THERESA J. VADNAIS; MARY ANN DEMARIA; AN ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 648 KB

Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescenceactivated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we