A comparison of cAMP phosphodiesterases in normal, malignant, and somatic cell hybrids

✍ Scribed by S. R. Ayad; J. F. Wright

• Book ID: 104783744
• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 776 KB
• Volume: 15
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00483994

✦ Synopsis

Hybrids (PCM) between a malignant mouse lymphoma suspension cell line (P388F-36) and a normal Chinese hamster fibroblastic cell line (Ch23) have already been isolated in this laboratory. Investigations were carried out on the cAMP phospodiesterases of the parents and two of these hybrids--PCM2 and PCM3. PCM3 shows a rather unusual growth characteristic in that a considerable proportion of the cells exist at any one time either in suspension or only loosely attached to the substratum, the remaining cell population existing in a monolayer form. It was found that each cell line exhibited multiple forms of the enzyme with varying affinities for cAMP. Both parents, although different, contained high-, low-, and extra-high apparent Km forms of the enzyme. The hybrids exhibited characteristics of both parental systems but were different from each other. Neither hybrid exhibited a high-Km enzyme, but both exhibited two low-Km forms. There was also a slight variation between monolayer and suspension cells of PCM3 hybrid. An attempt has been made to explain these phenomena with respect to hybridization and the growth characteristics of the cells.