In the synthesis of large peptides or proteins, highly homogeneous segments are indispensable for a convergent strategy either on a solid-phase resin or in solution. Employing Boc/Bzl chemistry to prepare fully protected segments with a free h-carboxyl group from the solid support, base-labile linke
A Combined Solid-Phase and Solution Strategy for Chemical Synthesis of Human Leptin
β Scribed by Yuji Nishiuchi; Tatsuya Inui; Hideki Nishio; Terutoshi Kimura
- Publisher
- John Wiley and Sons
- Year
- 2003
- Weight
- 57 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0931-7597
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β¦ Synopsis
Abstract
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π SIMILAR VOLUMES
with K-selectride to afford the desired hydroxy compound 24. Desilylation of 24 with HF ' py gave the hydroxy oxime 25. The C=N bond of 25 was reduced with NaBH,CN providing the desired product 26 together with its C4 epimer.[131 Finally, chromatographically purified 26 was deprotected to the target
A convenient solid-phase synthesis of oligonucleotides conjugated at the 3Π-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked