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A column radioassay for the quantification of vitamin B-12

โœ Scribed by R.C. Boguslaski; C.P. Rathjen


Book ID
115820789
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
487 KB
Volume
62
Category
Article
ISSN
0009-8981

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Under conditions of vitamin B12 deficiency the marine phytoplankton Thalassiosira pseudonana secretes into the growth medium a protein (Gm protein) that binds B12 specifically with an affinity constant of 2 x 10(11) M(-1). When Gm protein was used as a B12-specific binder in radioassays for the dire