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Application of competitive ligand binding for the radioassay of vitamin B12 and folic acid

โœ Scribed by Sheldon P. Rothenberg

Book ID
118947282
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
659 KB
Volume
22
Category
Article
ISSN
1532-8600

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Under conditions of vitamin B12 deficiency the marine phytoplankton Thalassiosira pseudonana secretes into the growth medium a protein (Gm protein) that binds B12 specifically with an affinity constant of 2 x 10(11) M(-1). When Gm protein was used as a B12-specific binder in radioassays for the dire