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A colorimetric procedure for the determination of γ-carboxyglutamic acid

✍ Scribed by Laura Pecci; Doriano Cavallini

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
450 KB
Volume
118
Category
Article
ISSN
0003-2697

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✦ Synopsis

A calorimetric method for the determination of y-carboxyglutamic acid (Gla) is presented. The procedure is based on the following results. (a) Gla is quantitatively converted into a proline derivative by reaction with acetaldehyde.

(b) This derivative is spectrophotometrically detected by the secondary amine reagent: nitroprusside and acetaldehyde in alkaline medium. Under the reported conditions, Beer's law is obeyed for concentrations of Gla varying from 0.5 to 5 X 1O-4 M. The method has been used to determine the Gla content in urine samples.

' This paper is dedicated to Professor Alessandro Rossi Fanelli on the occasion of his 75th birthday.

* Abbreviation used: Gla, y-carboxyglutamic acid.

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