✦ LIBER ✦

A colorimetric enzyme-linked on-bead assay for identification of synthetic substrates of protein tyrosine kinases

✍ Scribed by Scott E. Martin; Blake R. Peterson

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 171 KB
Volume: 8
Category: Article
ISSN: 1075-2617
DOI: 10.1002/psc.376

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✦ Synopsis

Protein tyrosine kinases play key roles in the progression of numerous human diseases including several types of cancers. We report here a simple colorimetric assay for tyrosine kinase activity employing synthetic peptide substrates prepared on Tentagel synthesis beads. Phosphorylation of compounds on beads was detected with an antiphosphotyrosine antibody complexed with a secondary antibody-alkaline phosphatase conjugate. This assay may prove useful for the identification and characterization of synthetic substrates of this important class of enzymes.

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