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High-Throughput Identification of Substrate Specificity for Protein Kinase by Using an Improved One-Bead-One-Compound Library Approach

✍ Scribed by Yun-Gon Kim; Dong-Sik Shin; Eun-Mi Kim; Hyung-Yeon Park; Chang-Soo Lee; June-Hyung Kim; Bon-Su Lee; Yoon-Sik Lee; Byung-Gee Kim


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
301 KB
Volume
119
Category
Article
ISSN
0044-8249

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A method is described for the elucidation of the peptide substrate phosphorylation specificity of a protein kinase. Peptide libraries with two to six degenerate positions and a length of seven or nine amino acids were generated directly on Sepharose beads by solidphase peptide synthesis according to