𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Standardization of an Enzyme-Linked Immunosorbent Assay for the Determination of Protein Tyrosine Kinase Activity

✍ Scribed by B. Schraag; G.E.J. Staal; S.S. Adriaansenslot; M. Salden; G. Rijksen

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
532 KB
Volume
211
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Description of an enzyme-linked immunoso
Description of an enzyme-linked immunosorbent assay for the detection of protein tyrosine kinase
✍ Iciar LΓ‘zaro; Miguel Gonzalez; GarbiΓ±e Roy; Luisa M. Villar; Pedro Gonzalez-Porq πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 415 KB

A solid immunoassay for the detection of protein tyrosine kinases has been developed. It is based on the binding of the synthetic polypeptide poly(Glu.Na,Tyr) 4:1 to microELISA wells, where the phosphorylation reaction takes place in the presence of ATP and enzyme. The phosphorylated tyrosine residu

Development of solid-phase enzyme-linked
Development of solid-phase enzyme-linked immunosorbent assays for the determination of epidermal growth factor receptor and pp60c-src tyrosine protein kinase activity
✍ K. Farley; H. Mett; E. McGlynn; B. Murray; N.B. Lydon πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 447 KB

Solid-phase ELISAs for the determination of EGF receptor (EGF-R) and pp60c-src tyrosine protein kinase activity are described. The methods were developed and optimized using purified recombinant EGF-R intracellular domain (ICD) and pp60c-src tyrosine protein kinases. A standardized assay that utiliz

The development of an enzyme-linked immu
The development of an enzyme-linked immunosorbent assay for the determination of cortisol binding globulin.
✍ A.W. van Rijswijk; J.C. Morgenthal; J.J.F. Taljaard πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 243 KB

## Abstract A simple, reliable and reproducible enzyme‐linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The e