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The development of an enzyme-linked immunosorbent assay for the determination of cortisol binding globulin.

✍ Scribed by A.W. van Rijswijk; J.C. Morgenthal; J.J.F. Taljaard

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
243 KB
Volume
17
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

A simple, reliable and reproducible enzyme‐linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The excellent agreement (r= 0.98) seen between the present study and a binding assay indicates that the polyclonal antibodies used recognize only intact steroid‐binding CBG. The intra‐ and inter‐assay coefficient of variation (4.0% and 7.1% respectively) compared favourably with those reported by other authors.

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