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A chromatographic and fluorimetric method for the determination of oestriol in pregnancy urine

✍ Scribed by Ruth Dvir; R. Chayen

Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
906 KB
Volume
45
Category
Article
ISSN
1873-3778

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✦ Synopsis

A method is described for the measurement of oestriol in pregnancy urine. After hydrolysis of the urine, oestrogens are reacted with r-dimethylaminonaphthalene-5sulphonyl chloride (DANSYL-chloride)

to give fluorescent derivatives. The oestriol derivative is separated by thin-layer chromatography on Kieselgel G in a solvent of ethanol-chloroform

(5 :gs). It is located by viewing under UV light, eluted and measured in a spectrofluorimeter, using wavelengths of 346 rnp and 525 m,u, respectively, for activation and emission. . . . .

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