𝔖 Bobbio Scriptorium
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A CHEMICAL ASSAY METHOD FOR PENICILLIN G

✍ Scribed by Sheehan, John C.; Mader, W. J.; Cram, Donald J.


Book ID
121282038
Publisher
American Chemical Society
Year
1946
Tongue
English
Weight
147 KB
Volume
68
Category
Article
ISSN
0002-7863

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A simple, highly sensitive, and rapid assay for highthroughput screening of penicillin G acylase-producing bacteria is presented. The method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin G acylase. T