A simple, highly sensitive, and rapid assay for highthroughput screening of penicillin G acylase-producing bacteria is presented. The method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin G acylase. T
Rapid continuous colorimetric enzyme assay for penicillin G acylase
โ Scribed by Howard Simons; Tim D. Gibson
- Book ID
- 110271466
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 31 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0951-208X
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