A cell line (LIM 2463) derived from a tubulovillous adenoma of the rectum

✍ Scribed by Robert H. Whitehead; Pauline van Eeden; Robyn E. Lukeis

• Book ID: 102868814
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 566 KB
• Volume: 48
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910480511

✦ Synopsis

We describe a new epithelial cell line (LIM 2463) derived from tubulovillous adenoma of the rectum. The cells grow as organoids and secrete large amounts of mucus. The cells are polarized, with a microvillar brush border, and express dipep-tidy1 peptidase IV in a polarized manner. No staining was seen with 3 other antibodies directed against other brush-border hydrolases or disaccharidases. Focal polarized staining was obtained with 2 antibodies directed against other brushborder-associated peptides. The cells are aneuploid with a distinctive karyotype (48, XX, +9, + 13). All attempts to clone the cells in semi-solid agar or to grow them as xenografts in nude athymic mice have failed.