A BRAIN-SPECIFIC PROTEIN WITH AFFINITY FOR DNA
β Scribed by N. Miani; A. Caniglia; V. Panetta
- Book ID
- 111169240
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 1021 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
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We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein
An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o