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Affinity Capture of Specific DNA-Binding Proteins for Mass Spectrometric Identification

✍ Scribed by Yaneva, Mariana; Tempst, Paul


Book ID
120930234
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
311 KB
Volume
75
Category
Article
ISSN
0003-2700

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We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble

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We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein