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A Bacterial Type III Secretion Assay for Delivery of Fungal Effector Proteins into Wheat

โœ Scribed by Upadhyaya, Narayana M.; Mago, Rohit; Staskawicz, Brian J.; Ayliffe, Michael A.; Ellis, Jeffrey G.; Dodds, Peter N.


Book ID
126863499
Publisher
The American Phytopathological Society
Year
2014
Tongue
English
Weight
829 KB
Volume
27
Category
Article
ISSN
0894-0282

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added to the dsDNA before hybridization. The color of the solution in test reaction was compared with the set of standards and the presence of dsDNA was determined. The setup is able to detect picogram level of dsDNA and colorimetric method is found tenfold more sensitive then visualized under UV tr