Direct delivery of bacterial type III secretion system effector proteins to the eukaryotic cell as a tool to study bacterial pathogenesis
✍ Scribed by A. Jalili; J. Atherton; R. Delahay
- Publisher
- Elsevier
- Year
- 2009
- Tongue
- English
- Weight
- 69 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1871-6784
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✦ Synopsis
added to the dsDNA before hybridization. The color of the solution in test reaction was compared with the set of standards and the presence of dsDNA was determined. The setup is able to detect picogram level of dsDNA and colorimetric method is found tenfold more sensitive then visualized under UV trans-illuminator. The advantages of this method are (1) Isothermal amplification does not require thermal cycler. (2) For the visualization of the amplified fragment restriction digestion and gel electrophoresis is not required. (3) Less instrumentation hence carried out on site. (4) Cost effective and operated with formal training. (5) Rapid detection of pathogen within 60 min. (6) The pathogenic load can be determined visually. The array is being developed to detect a range of pathogens. In future this technology will also be utilized to detect genetically modified seeds and bioterrorism.