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A colorimetric assay for studying effector secretion through the bacterial type III secretion system

✍ Scribed by Masami Miyake; Sadatsugu Sakane; Chiho Kobayashi; Miyuki Hanajima-Ozawa; Aya Fukui; Shigeki Kamitani; Yasuhiko Horiguchi

Book ID
109329968
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
216 KB
Volume
278
Category
Article
ISSN
0378-1097

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✍ A. Jalili; J. Atherton; R. Delahay πŸ“‚ Article πŸ“… 2009 πŸ› Elsevier 🌐 English βš– 69 KB

added to the dsDNA before hybridization. The color of the solution in test reaction was compared with the set of standards and the presence of dsDNA was determined. The setup is able to detect picogram level of dsDNA and colorimetric method is found tenfold more sensitive then visualized under UV tr