𝔖 Bobbio Scriptorium
✦   LIBER   ✦

749 Determination of the amino acid sequence and cDNA cloning of the major allergen of the domestic cat,

✍ Scribed by MORGENSTERN, J; GRIFFITH, I; BRAUER, A; POLLOCK, J; ROGERS, B; YU, X; BURKE, C; CHAPMAN, M; KUO, M

Book ID
119060930
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
132 KB
Volume
87
Category
Article
ISSN
1097-6825

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Apple Allergy: The cDNA Sequence of the
Apple Allergy: The cDNA Sequence of the Major Allergen of Apple, Determined by Performing PCR with a Primer Based on theN-Terminal Amino Acid Sequence, is Highly Homologous to the Sequence of the Major Birch Pollen Allergen
✍ SchΓΆning, Britta; Ziegler, Wolfgang H; Vieths, Stefan; Baltes, Werner πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 748 KB

Considering the known N-terminal amino acid sequence of the major apple allergen, a polymerase chain reaction (PCR) primer was selected to amplify cDNA encoding this protein. A single PCR product was obtained, cloned into Escherichia coli and subsequently sequenced. The missing 5'-end of the apple c