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Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1

✍ Scribed by Pastorello, Elide A.; Ortolani, Claudio; Baroglio, Chiara; Pravettoni, Valerio; Ispano, Marco; Giuffrida, Maria Gabriella; Fortunato, Donatella; Farioli, Laura; Monza, Mara; Napolitano, Lorenzo; Sacco, Marco; Scibola, Elisabetta; Conti, Amedeo

Book ID
120613949
Publisher
Walter de Gruyter GmbH & Co. KG
Year
1999
Tongue
English
Weight
173 KB
Volume
380
Category
Article
ISSN
1431-6730

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πŸ“œ SIMILAR VOLUMES

Apple Allergy: The cDNA Sequence of the
Apple Allergy: The cDNA Sequence of the Major Allergen of Apple, Determined by Performing PCR with a Primer Based on theN-Terminal Amino Acid Sequence, is Highly Homologous to the Sequence of the Major Birch Pollen Allergen
✍ SchΓΆning, Britta; Ziegler, Wolfgang H; Vieths, Stefan; Baltes, Werner πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 748 KB

Considering the known N-terminal amino acid sequence of the major apple allergen, a polymerase chain reaction (PCR) primer was selected to amplify cDNA encoding this protein. A single PCR product was obtained, cloned into Escherichia coli and subsequently sequenced. The missing 5'-end of the apple c