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587 Amino acid sequence analysis and cloning of the major peanut allergen, Ara h II

✍ Scribed by STANLEY, J; BURKS, A; COCKRELL, G; HELM, R; BANNON, G


Book ID
119495910
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
95 KB
Volume
97
Category
Article
ISSN
1097-6825

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Considering the known N-terminal amino acid sequence of the major apple allergen, a polymerase chain reaction (PCR) primer was selected to amplify cDNA encoding this protein. A single PCR product was obtained, cloned into Escherichia coli and subsequently sequenced. The missing 5'-end of the apple c