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6-Hydroxy-D-nicotine oxidase of Arthrobacter oxidans : Gene structure of the flavoenzyme and its relationship to 6-hydroxy-L-nicotine oxidase

✍ Scribed by Roderich BRANDSCH; Ari E. HINKKANEN; Ludwig MAUCH; Heiner NAGURSKY; Karl DECKER


Book ID
115125212
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
616 KB
Volume
167
Category
Article
ISSN
1432-1327

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Plasmid pAO1 of Arthrobacter oxidans enc
✍ Brandsch, Roderich ;Faller, Waltraud ;Schneider, Klaus πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 815 KB

The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of th