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Crystal Structure of 6-Hydroxy-d-nicotine Oxidase from Arthrobacter nicotinovorans

✍ Scribed by Jochen W.A. Koetter; Georg E. Schulz


Book ID
116662357
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
599 KB
Volume
352
Category
Article
ISSN
0022-2836

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The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of th