In vivo and in vitro expression of the 6-hydroxy-D-nicotine oxidase gene of Arthrobacter oxidans, cloned into Escherichia coli, as an enzymatically active, covalently flavinylated polypeptide

Plasmid pAO1 of Arthrobacter oxidans enc

Plasmid pAO1 of Arthrobacter oxidans encodes 6-hydroxy-d-nicotine oxidase: cloning and expression of the gene in Escherichia coli

✍ Brandsch, Roderich ;Faller, Waltraud ;Schneider, Klaus 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 815 KB

The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of th