4693966 Human monoclonal antibodies from lymphocytes of patients with malignant melanoma
β Scribed by Lillie, Jana
- Book ID
- 122197991
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 67 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0734-9750
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Two conceptually unique approaches are currently available for the production of human monoclonal antibodies - the 'hybridoma' technique, based on the fusion of antibody-producing B lymphocytes with plasmacytoma cells or lymphoblastoid cell lines; and the use of Epstein-Barr virus (EBV) to 'immortal
The hybridoma technique was used to generate monoclonal antibodies against a wide spectrum of melanoma-associated surface antigens. Mice were immunized against the human melanoma lines , which differ with respect to a number of biological and biochemical properties. Spleen cells were fused with P3x6