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4563305 Radiolabelled substrates for assaying mammalian enzymes


Book ID
123454616
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
67 KB
Volume
4
Category
Article
ISSN
0734-9750

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A sensitive microplate assay for the det
โœ B.D. Robertson; G.E. Kwan-Lim; R.M. Maizels ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 365 KB

A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By m