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Multiple-substrate enzyme assays: a useful approach for profiling enzyme activity in soils?

โœ Scribed by Michael Stemmer

Book ID
113909874
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
240 KB
Volume
36
Category
Article
ISSN
0038-0717

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Enzyme Kinetics Determined Using Calorim
Enzyme Kinetics Determined Using Calorimetry: A General Assay for Enzyme Activity?
โœ Matthew J. Todd; Javier Gomez ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 119 KB

Two techniques for determining enzyme kinetic constants using isothermal titration microcalorimetry are presented. The methods are based on the proportionality between the rate of a reaction and the thermal power (heat/time) generated. (i) An enzyme can be titrated with increasing amounts of substra