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[35S]GTPγS binding stimulated by endomorphin-2 and morphiceptin analogs

✍ Scribed by Jakub Fichna; Jean-Claude do-Rego; Piotr Kosson; Peter W. Schiller; Jean Costentin; Anna Janecka

Book ID
116292839
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
199 KB
Volume
345
Category
Article
ISSN
0006-291X

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Subtype-specific stimulation of [35S]GTPγS binding by recombinant α2-adrenoceptors
✍ Juha M. Peltonen; Marjo Pihlavisto; Mika Scheinin 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 99 KB

We measured agonist-stimulated binding of the stable GTP-analog guanosine-5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS) as a functional assay to monitor G-protein activation by recombinant human alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpha2C. (-)-Noradrenaline was a full agonist in all