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3′ BCR recombines with IGL locus in BCR-ABL–positive Philadelphia-negative chronic myeloid leukemia

✍ Scribed by Suzanne M. Benjes; Lynn J. Millow; Aaron R. Jeffs; Stephen J. Sowerby; Anthony E. Reeve; Christine M. Morris

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
235 KB
Volume
26
Category
Article
ISSN
1045-2257

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✦ Synopsis

We have isolated the 3Ј BCR breakpoint junction of a complex BCR-ABL1 rearrangement found in leukemic cells with a cytogenetically normal karyotype, and the corresponding germline fragment that spanned the 3Ј BCR recombination site. Fluorescence in situ hybridization localized the 3Ј BCR recombination site to 22q11, about 350-600 kb proximal to BCR. Restriction map and DNA sequence comparisons indicated that 3Ј M-Bcr had recombined at a site within the variable region (Itv Region IV) of the immunoglobulin lambda (IGL) locus. Somatic rearrangement of DNA sequences (variable, joining, and constant regions) within the IGL locus, as in other Ig and TCR loci, represents the basis for human antibody diversity. Misrecombination of these somatically rearranging sites has been associated with chromosomal rearrangements in lymphoid leukemia and lymphoma, but there are no previous descriptions of IGL involvement in genomic aberrations associated with myeloid leukemia.

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