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15N-NMR spectroscopy. IV. Comparison of poly(L-lysine) and isopoly(L-lysine)

✍ Scribed by William E. Hull; Hans R. Kricheldorf; Martin Fehrle

Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
873 KB
Volume
17
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The natural abundance ^15^N‐nmr spectroscopy has been used to characterize the isomeric polymers (L‐Lys)~n~ and iso (L‐Lys)~n~ in aqueous solution. Although the peptide nitrogens of the two polymers have nearly equivalent shifts at pH < 10, the amino nitrogens differ by 5–6 ppm at pH < 7 and provide an easy means of identification. Furthermore, the polymers are distinguishable by the pK~a~ of the amino group and the basicity of the peptide nitrogen. At pH 10.3 and 25°C, (Lys)~n~ exhibits line broadening and an upfield chemical shift of the peptide nitrogen, indicative of the coil → helix transition. The formation of 100% helix may produce a shift as large as 5 ppm, which probably makes ^15^N‐nmr spectroscopy more suitable for studies of this transition.

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