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β-Galactosidase transferase activity in ice and use of vinyl-β-d-galactoside as donor

✍ Scribed by V. Chiffoleau-Giraud; P. Spangenberg; C. Rabiller

Book ID: 104361211
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 420 KB
Volume: 8
Category: Article
ISSN: 0957-4166
DOI: 10.1016/s0957-4166(97)00194-8

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✦ Synopsis

The ability of vinyl-13-D-galactoside as a donor in transglycosylation reactions catalysed by the [~-glycosidase from Aspergillus oryzae using allylic alcohol and methylct-galactoside as acceptors is tested. A kinetic study made in comparison with another donor, the 2-nitrophenyl-13-D-galactoside, shows that the use of the latter leads at room temperature to better yields than those obtained from the former. A reverse situation is observed in ice at -7°C, conditions in which the yield for transglycosylation can be enhanced from 59% to 82% with methyl-ot-galactoside as an acceptor. (~) 1997 Elsevier Science Ltd

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