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α(1,3)Fucosyltransferase expression in E-selectin-mediated binding of gastrointestinal tumor cells

✍ Scribed by Bianca M. Wittig; Rüdiger Thees; Hildegard Kaulen; Karin Gott; Eckart Bartnik; Clemens Schmitt; Karl-Hermann Meyer Zum Büschenfelde; Wolfgang Dippold

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
634 KB
Volume
67
Category
Article
ISSN
0020-7136

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✦ Synopsis

E-selectin recognizes the oncofetal antigen sialyl-Lewis X, which is highly expressed in adenocarcinoma. Five cu(l,3)fucosyltransferases (FT) have been cloned that confer cell-surface expression of sialyl-Lewis X on transfected cells. We show here that I2/ I8 gastrointestinal-tumor cell lines bind specifically to immobilized E-selectin and that in sialyl-Lewis-X-positive cells binding is inhibited with a monoclonal antibody against sialyl-Lewis X. Using RT-PCR, we determined the expression of the dl ,3)fucosyltransferases 111, IV, V, VI and Vll in gastrointestinal tumor cells. Transcripts of FT IV and FT VII are abundantly expressed in all tested cells. Therefore no single fucosyltransferase could be correlated with the expression of sialyl-Lewis X and the ability of the tumor cells to bind to E-selectin.

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