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α1-Antitrypsin in serum determined by capillary isoelectric focusing

✍ Scribed by Anna Lupi; Simona Viglio; Maurizio Luisetti; Marina Gorrini; Pierpaolo Coni; Gavino Faa; Giuseppe Cetta; Paolo Iadarola

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
183 KB
Volume
21
Category
Article
ISSN
0173-0835

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✦ Synopsis

1 -Antitrypsin in serum determined by capillary isoelectric focusing

A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5±5.0 range has been developed for the identification of alpha 1 -antitrypsin (a 1 AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose a 1 AT phenotype was previously identified and of twelve subjects whose a 1 AT phenotype was unknown. The results obtained allowed us to confirm or identify the a 1 AT phenotype in all sera tested. This procedure seems particularly suitable for identification of a 1 AT variants associated with diseases of clinical relevance.

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