Peak identification in capillary isoelectric focusing using the concept of relative peak position as determined by two isoelectric point markers
✍ Scribed by Jiaqi Wu; Tiemin Huang
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 289 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
In CIEF analysis, sample peaks can be identified by their relative peak positions (RPP) that are determined using only two internal p__I__ markers. The two internal p__I__ marker peaks should bracket, as close as possible, the sample peaks. The RPP values of the sample peaks are then calculated using the p__I__ values, peak positions of the two p__I__ markers, and peak position of the sample. Use of this method can effectively compensate for pH gradient distortions that often occur as a result of salts. Also, as shown by the results of this paper, regardless of the linearity of the pH gradient established by the given carrier ampholytes, sample peaks can be identified within an SD of 0.1 pH unit in RPP (<2% RSD) as long as the sample is run using the same carrier ampholytes and maintaining salt concentrations in the range of 0–15 mM.