α-Mannosidases from suspension-cultured sugar beet cells
✍ Scribed by Hiroshi Masuda; Shinobu Narita; Shiro Sugawara
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 631 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0031-9422
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Previous methods of extracting pectin from sugar-beet have used pulp as the starting material. As the temperature and pressure of the pulping process may modify the architecture of the cell wall, we have adapted a relatively non-disruptive method to characterise cell wall material (CWM) isolated dir
## Abstract Three types of α‐D‐mannosidase have been found in L1210 cells, a lysosomal one with an optimum activity at pH 4; a soluble one, thermosensitive, with an optimum activity at pH 6, and one with an optimum activity at pH 5.25, probably related to the Golgi apparatus.