Acid hydrolases are synthesized as precursors that undergo several posttranslational modifications including proteolytic processing to a smaller mature enzyme. The amount of proteolytic processing varies for different acid hydrolases, and many details of the intracellular pathways are not known. The
α-L-fucosidase secretion by human embryonic skin fibroblasts
✍ Scribed by I. D. Belyaeva; T. S. Ivleva; G. Ya. Vidershain
- Publisher
- Springer US
- Year
- 1986
- Tongue
- English
- Weight
- 386 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0007-4888
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Human a-L-fucosidase, purified from placenta, was taken up from the culture medium by skin fibroblasts from patients with fucosidosis (a-L-fucosidase deficiency). The rate of uptake was low (uptake coefficient = 6 x lo-\* ml.mg-l.h-\*). Intracellular a-L-fucosidase activity was directly proportiona
Human placental a-L-fucosidase has been purified 670-fold with a 57% yield by a one-step affinity chromatographic procedure using agaroseepsilon-amino-caproyl-fucosamine. 424
## Abstract We have studied some aspects of the regulation of hyaluronic acid synthesis in cultured human skin fibroblasts derived from normal individuals. In these cells 85% of radiolabeled glucosamine is incorporated into two types of macromolecules, hyaluronic acid and glycoproteins, characteriz