Purification of human placental α-l-fucosidase by affinity chromatography
✍ Scribed by Jack A. Alhadeff; A.L. Miller; John S. O'Brien
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 681 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Human placental a-L-fucosidase has been purified 670-fold with a 57% yield by a one-step affinity chromatographic procedure using agaroseepsilon-amino-caproyl-fucosamine. 424
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Human a-L-fucosidase, purified from placenta, was taken up from the culture medium by skin fibroblasts from patients with fucosidosis (a-L-fucosidase deficiency). The rate of uptake was low (uptake coefficient = 6 x lo-\* ml.mg-l.h-\*). Intracellular a-L-fucosidase activity was directly proportiona
A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L