α-Amylase production byBacillus amyloliquefaciensin a membrane recycle bioreactor
✍ Scribed by Caroline Morcel; Claus Emborg; Søren Madsen; K. Biedermann
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 293 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
A strain of Bacillus amyloliquefaciens was cultivated in a membrane recycle bioreactor for the production of an extracellular or-amylase. Continuous cultivations of B. amyloliquefaciens in the recycle fermentor led to higher cell mass and volumetric productivities than the ones obtained in batch or chemostat cultivations; the or-amylase activities were lower than in the batch mode but significantly higher than in the chemostat mode. The operation of the membrane recycle bioreactor was sometimes disturbed by high broth viscosity leading to a stronger fouling of the membrane.
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Using a totally defined synthetic medium the effect of lactose and nitrogen on eel physiology and or-amylase production by Bacillus amyloliquefaciens B155 were investigated. Results showed cell growth and or-amylase production patterns to be similar regardless of the limiting nutrient and suggested