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Yeast two-hybrid system: part B—screening procedure

✍ Scribed by Debra L. Parchaliuk; Robert D. Kirkpatrick; Sharon L. Simon; Ronald Agatep; R. Daniel Gietz


Book ID
114393094
Publisher
Elsevier
Year
1999
Weight
154 KB
Volume
4
Category
Article
ISSN
1366-2120

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The yeast two-hybrid system is a genetic method that detects protein-protein interactions. One application is the detection by library screening of new interactors of a protein of known function. In the August issue of Nature Genetics, Fromont-Racine et al. 1 showed for the first time that the const