𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The yeast two-hybrid system

✍ Scribed by József Szeberényi


Publisher
The American Society for Biochemistry and Molecular Biology
Year
2006
Tongue
English
Weight
152 KB
Volume
34
Category
Article
ISSN
1470-8175

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Two-hybrid systematic screening of the y
✍ Nicolas Lecrenier; Françoise Foury; Andre Goffeau 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 84 KB 👁 2 views

The yeast two-hybrid system is a genetic method that detects protein-protein interactions. One application is the detection by library screening of new interactors of a protein of known function. In the August issue of Nature Genetics, Fromont-Racine et al. 1 showed for the first time that the const

In vivo construction of cDNA libraries f
✍ Fusco, Carlo; Guidotti, Elena; Zervos, Antonis S. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 190 KB 👁 2 views

We describe a simple and efficient one-step method to make cDNA libraries using homologous recombination in yeast. cDNA from any source, together with a linear vector, is used to transform yeast. Through homologous recombination and gap repair, the cDNA is unidirectionally incorporated into the yeas

A yEGFP-based reporter system for high-t
✍ Jun Chen; Jianhong Zhou; Weon Bae; Claire K. Sanders; John P. Nolan; Hong Cai 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 308 KB

## Abstract The yeast two‐hybrid (Y2H) assay is a widely used method to study protein–protein interactions, a major objective in postgenome research. Despite the tremendous utility of the Y2H assay, several issues including accuracy, speed, automation, and cost‐effectiveness limit its application i

Identification of FMRP-associated mRNAs
✍ Ke Zou; Jian Liu; Ning Zhu; Jianhong Lin; Qi Liang; W. Ted Brown; Yan Shen; Nanb 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 140 KB

## Abstract Fragile X syndrome, one of the most common forms of inherited mental retardation, results from the absence of the fragile X mental retardation protein (FMRP), which is encoded by the fragile X mental retardation gene 1 (__FMR1__). FMRP is an RNA‐binding protein involved in translational