The yeast two-hybrid system
✍ Scribed by József Szeberényi
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2006
- Tongue
- English
- Weight
- 152 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1470-8175
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We describe a simple and efficient one-step method to make cDNA libraries using homologous recombination in yeast. cDNA from any source, together with a linear vector, is used to transform yeast. Through homologous recombination and gap repair, the cDNA is unidirectionally incorporated into the yeas
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