Nucleosomal subunits isolated from rabbit thymus nuclei in 0.04 M K2SO 4-0.02 M Tris, pH 7.4 were devoid of histone H1, while whole chromatin prepared in the same buffer contained the full complement of histone HI. The question is asked why histone H1 dissociates from the subunits but not from the h
Yeast chromatin: Search for histone H1
β Scribed by Sommer, Andreas
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 846 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
β¦ Synopsis
Yeast chromatin, isolated by a rapid procedure contains in addition to histones H2A, H2B, H3 and H4 a fifth major basic protein. This fifth polypeptide is not an intrinsic component of the nucleosome structure. It has properties of both histone and nonhistone proteins and might represent an early form of histone H1 and of high mobility group nonhistone proteins of higher eukaryotes. Electron microscopic visualization of isolated yeast nucleosomes substaniates further the similarity of the chromatin structudre of this unicellular eukaryote to that of higher eukaryotes.
π SIMILAR VOLUMES
We have investigated using the DNA binding fluorochromes 7-aminoactinomycin (7-AAMD) and 48,6diamidino-2-phenylindole (DAPI) as cytochemical probes for linker histone (H1)-chromatin interactions in situ. Human lymphocytes, permeabilized with digitonin, were exposed to increasing concentrations of so