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On the binding of histone H1 in chromatin

✍ Scribed by Robert C. Krueger

Publisher
Springer
Year
1986
Tongue
English
Weight
448 KB
Volume
11
Category
Article
ISSN
0301-4851

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✦ Synopsis

Nucleosomal subunits isolated from rabbit thymus nuclei in 0.04 M K2SO 4-0.02 M Tris, pH 7.4 were devoid of histone H1, while whole chromatin prepared in the same buffer contained the full complement of histone HI. The question is asked why histone H1 dissociates from the subunits but not from the high molecular weight material. We propose that, at physiological salt concentrations, histone H1 is not bound to linker DNA as depicted in the current models; rather, alternate attachment sites, present only in the polymer, are involved.

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